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. 2006 May 15;203(5):1197–1207. doi: 10.1084/jem.20060418

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Copyright © 2006, The Rockefeller University Press

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Figure 8. — Vβ repertoire changes in mice expressing high or low levels of CD1d. (top) Levels of CD1d expression (mean fluorescence intensity indicated above histograms) by thymocytes of indicated mice. CD1d ^−/LE are mice with a null (−) allele and a low expression (LE) allele the result of a loxP insertion in the 5′UTR of CD1d1. pLck/high are mice with high level expression of a CD1d transgene driven by the proximal Lck promoter. (bottom) Percentages of Vβ7 and Vβ8 cells among α-GalCer-CD1d tetramer⁺ cells were determined by FACS analysis of indicated mice. Bars represent mean and SD (white, Vβ7; gray, Vβ8) based on indicated “n” number of mice examined. “CD1d =”: relative CD1d expression level (mean fluorescence intensity) compared with B6 WT mice; “NKT =”: relative numbers of NKT thymocytes compared with B6 WT mice. *, statistical significance (P < 0.05) using unpaired Student's t test.