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. 2006 May 15;203(5):1273–1282. doi: 10.1084/jem.20052205

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — Immunization with antigen-activated DCs leads to proliferation of adventitial T lymphocytes within the aorta. CD40L-activated DCs were pulsed with antigen peptide and either 10⁵ bone marrow–derived DCs in 200 μl PBS or 200 μl PBS were injected i.v. into OT-1 transgenic recipient mice. At 48 and 60 h, 1 mg BrdU was injected i.p. Aorta, pLN, spleen, and blood were collected 72 h after transfer, and the cell suspensions were stained with anti-CD45, -CD8, -CD44, and anti-BrdU Abs. All plots were gated on CD45⁺ lymphocyte gate. (A) Representative plots from one experiment. The numbers in quadrants represents BrdU⁺ cells. (B) The percentage of BrdU⁺ CD8⁺ T cells in the aortas and spleens of OT-1 control (gray bars) or immunized OT-1 recipient mice (black bars) are presented. Results mean ± SE (n = 8). **, P < 0.01.