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. Author manuscript; available in PMC: 2007 Dec 7.
Published in final edited form as: J Immunol. 1993 Aug 1;151(3):1410–1418.

Table II.

HLA-A2.1 restricted melanoma specific TIL can specifically lyse vac-A2 infected melanoma cell linesa

Expt. 1
Expt. 2
Target Vaccinia HLA-A2 TIL660 TIL1074 Kd-CTL Target Vaccinia HLA-A2 TIL660 TIL620
% specific lysis % specific lysis
501mel None + 29 24 ND 501mel None + 45 48
928mel None − −3 0 ND 888mel None − 14 7
kDa − −2 −3 67 kDa − 6 2
β2m − 5 1 ND β2m ND ND ND
A2 + 13 18 1 A2 + 28 19
A2, β2m + 24 34 −3 A2, β2m + 28 28
G361 None − 4 2 ND G361 None − 7 2
kDa − 7 9 47 kDa − 6 2
A2 + 28 30 −2 A2 + 30 22
A2, β2m + ND ND ND A2, β2m + 32 28
a

HLA-A2 negative melanoma lines 928mel, 888mel, and G361 were infected with vac-A2, then tested for lysis by the HLA-A2-restricted TIL 660 and 1074. A 5-h 51Cr release assay was performed. Results are shown as % specific lysis at E:T = 40:1. HLA-A2 surface expression was tested by flow cytometry. Only cells infected with vac-A2 were lysed, and some increase was seen in lysis upon coinfection by vac-A2 and vac-β2m. 501mel is an HLA-A2+ melanoma control, and Kd-CTL are H-2Kd-restricted vaccinia viral Ag-specific murine CTL. Lysis of 888mel by TIL660 in experiment 2 (14%) was not statistically significant because of the high standard deviation of the data. Bold numbers, Statistically significant lysis (p < 0.05).