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Time course of oligonucleotide excision observed when a site-directed selenophenyl or inverted C4′ backbone modification were incorporated within sites of one (lanes 1, 4, and 7) or three mispaired bases (lanes 2, 5, and 8). Control reactions contained substrates with the three mispaired bases but no C4′ backbone lesion (lanes 3, 6, and 9). Only the bottom part of the gel is shown.
