FIG. 3.

Hybrid-ITR vector genomes enhance directional heterodimerization after infection of C57BL/6 Rag1-KO mouse anterior tibialis muscle. Directional genome heterodimerization was assessed after tibialis infection with the indicated viral combinations in the presence of Doxil. (A) β-Galactosidase enzymatic assays performed on tissue lysates 2 weeks postinfection. Data represent mean (+SEM) β-galactosidase activity of two to five independent infections for each group as indicated. (B) Morphometric quantification of X-Gal-stained mouse muscle from the indicated infections (left) and representative photomicrographs of the X-Gal-stained muscle sections (right). Data represents mean (+SEM) percent X-Gal-positive area observed in liver sections (n = 5 mice for each experimental group).