Fig. 6. isc1⊿ shows mitochondrial defects.

(A) Petite ratio of isc1⊿ . WT and isc1⊿ cells were inoculated at OD₆₀₀ of 0.1 in YPD medium, incubated at 39°C for the indicated times, and plated onto YPD plates. Small colonies that grew on YPD plates were replica plated onto YPGE plates to confirm the inability to grow on non-fermentable carbon medium, and the numbers of the colonies which grew on YPD but not on YPGE plates were counted. Statistical differences were evaluated by Student's unpaired t test and are indicated by asterisks (**p<0.01, *p<0.12). (B) Hydrogen peroxide sensitivity of isc1⊿. WT and isc1⊿ were treated with 1 mM hydrogen peroxide for 45 min, plated onto YPD and the formed colonies were counted. Statistical difference was evaluated by Student's unpaired t test and is indicated by asterisks (**p<0.01). (C) Ethidium bromide sensitivity of isc1⊿. WT and isc1⊿ cells were treated with 50 μM ethidium bromide, and 5 μl of the suspension was spotted onto a YPD plate with serial dilutions at 10 fold.