Table 1.

Adenovirus E4 ORF1 transforming proteins possess consensus PDZ domain-binding motifs at their C termini^*

PDZ domain-binding motif	Position
	−3  −2  −1  0
	Transforming activity†	X	(S/T)	X	(V/I)
9ORF1	+++++	A	T	L	V
mutIII-A	−	A	P
mutIII-B	−	A	T	L	V	SACFL
mutIII-C	+	D	T	L	V
mutIII-D	+	A	T	P	V
T123A	ND	A	A	L	V
T123D	ND	A	D	L	V
V125A	ND	A	T	L	A
12ORF1	+++++	A	S	L	I
3ORF1	+++++	A	T	M	I
5ORF1	+++++	A	S	N	V
APC	N/A	V	T	S	V

^*The four C-terminal amino acid residues of each wild-type protein are shown. Alterations of mutant 9ORF1 proteins are depicted as outlined amino acid residues. 

^†In vitro transforming activities of wild-type and mutant E4 ORF1 proteins (7, 15). +++++, strong transforming activity; +, weak transforming activity; −, no detectable transforming activity; ND, not done; N/A, not applicable.