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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: J Immunol Methods. 2007 Sep 12;328(1-2):97–105. doi: 10.1016/j.jim.2007.08.011

Figure 3. Monte Carlo simulation of L-selectin surface transport predicts cap formation during centrifugation.

Figure 3

(A) Fluid streamlines inside and outside of the neutrophil (modeled as a viscous fluid drop) during sedimentation. (B) 3-D plot showing the initial random distribution of L-selectin receptor in the Monte Carlo simulation of cell sedimentation. (C) Final surface distribution of L-selectin receptor after 50 seconds of sedimentation down 5 cm of test tube length at 280× g. (D) Final surface distribution of L-selectin receptor after 3.9 hours of sedimentation down 5 cm of test tube length at 1× g. Cell sedimentation is directed from top-to-bottom, producing an external flow directed from bottom-to-top when observed from a coordinate system moving with the cell center.