Table 3.
Flux ranges
| Relaxed modela,b |
Full model
|
Reduced model
|
Basic model
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Abbreviationc | Subsystem | min | max | min | max | min | max | min | max |
| ICL | Anaplerotic reactions | 0.0 | 12.3 | 0.0 | 1.0 | 0.0 | 0.4 | 0.0 | 0.0 |
| MALS | Anaplerotic reactions | 0.0 | 12.3 | 0.0 | 1.0 | 0.0 | 0.4 | 0.0 | 0.0 |
| ME1x | Anaplerotic reactions | 0.0 | 75.9 | 0.0 | 4.0 | 0.0 | 3.7 | 0.0 | 0.0 |
| PPA | Anaplerotic reactions | 0.8 | 129.4 | 0.8 | 107.2 | 0.8 | 80.1 | 0.8 | 83.1 |
| PPC | Anaplerotic reactions | 0.0 | 130.1 | 1.1 | 6.8 | 1.1 | 5.5 | 1.4 | 5.5 |
| PPCK | Anaplerotic reactions | 0.0 | 128.7 | 0.0 | 5.4 | 0.0 | 4.0 | 0.0 | 4.0 |
| ACONT | Citrate Cycle (TCA) | 0.5 | 12.8 | 0.8 | 4.3 | 0.9 | 3.2 | 0.9 | 3.1 |
| AKGD | Citrate Cycle (TCA) | 0.0 | 12.3 | 0.0 | 2.7 | 0.0 | 2.7 | 0.1 | 2.6 |
| CITL | Citrate Cycle (TCA) | 0.0 | 128.7 | 0.0 | 128.6 | 0.0 | 128.7 | 0.0 | 0.0 |
| CS | Citrate Cycle (TCA) | 0.5 | 141.0 | 0.8 | 109.4 | 1.4 | 82.4 | 0.9 | 3.1 |
| FRD2 | Citrate Cycle (TCA) | 0.0 | 68.7 | 0.0 | 58.6 | 0.0 | 0.0 | 0.0 | 0.0 |
| FRD3 | Citrate Cycle (TCA) | 0.0 | 68.7 | 0.0 | 58.6 | 4.0 | 41.5 | 0.0 | 0.0 |
| FUM | Citrate Cycle (TCA) | 0.4 | 281.5 | 1.0 | 230.0 | 0.7 | 14.3 | 0.8 | 5.5 |
| ICDHy | Citrate Cycle (TCA) | 0.5 | 12.8 | 1.1 | 3.2 | 0.5 | 3.2 | 0.9 | 3.1 |
| MDH | Citrate Cycle (TCA) | −59.1 | 281.5 | −1.4 | 230.0 | −1.1 | 14.3 | 0.8 | 5.5 |
| SUCD1i | Citrate Cycle (TCA) | 0.0 | 77.3 | 2.6 | 60.5 | 5.1 | 43.8 | 0.4 | 2.6 |
| SUCOAS | Citrate Cycle (TCA) | −12.3 | 18.4 | −4.0 | 0.1 | −2.6 | 11.6 | −2.5 | 0.0 |
| ENO | Glycolysis/Gluconeogenesis | −44.3 | 18.8 | −40.3 | 18.4 | 16.5 | 18.4 | 16.6 | 18.8 |
| FBA | Glycolysis/Gluconeogenesis | −0.3 | 9.6 | 0.2 | 9.6 | 7.7 | 9.6 | 7.9 | 9.6 |
| FBP | Glycolysis/Gluconeogenesis | 0.0 | 128.7 | 0.0 | 107.3 | 0.0 | 79.8 | 0.0 | 0.0 |
| G1PP | Glycolysis/Gluconeogenesis | 0.0 | 128.7 | 0.0 | 107.3 | 0.0 | 0.0 | 0.0 | 0.0 |
| GAPD | Glycolysis/Gluconeogenesis | −32.3 | 19.0 | −20.1 | 19.0 | 17.2 | 19.0 | 17.3 | 19.0 |
| GLCP | Glycolysis/Gluconeogenesis | 0.0 | 128.7 | 0.0 | 107.3 | 0.0 | 0.0 | 0.0 | 0.0 |
| GLCS1 | Glycolysis/Gluconeogenesis | 0.1 | 128.7 | 0.1 | 107.4 | 0.1 | 0.1 | 0.1 | 0.1 |
| GLGC | Glycolysis/Gluconeogenesis | 0.1 | 128.7 | 0.1 | 107.4 | 0.1 | 0.1 | 0.1 | 0.1 |
| HEX1 | Glycolysis/Gluconeogenesis | 0.0 | 136.2 | 0.0 | 115.9 | 0.0 | 10.0 | 0.0 | 10.0 |
| PDH | Glycolysis/Gluconeogenesis | 0.0 | 27.1 | 0.0 | 16.4 | 0.0 | 16.4 | 0.0 | 15.3 |
| PFK | Glycolysis/Gluconeogenesis | 0.0 | 138.3 | 0.0 | 116.4 | 7.8 | 88.3 | 7.9 | 9.6 |
| PGI | Glycolysis/Gluconeogenesis | 0.0 | 9.9 | 0.0 | 9.9 | 4.4 | 9.9 | 4.7 | 9.9 |
| PGK | Glycolysis/Gluconeogenesis | −32.3 | 19.0 | −20.1 | 19.0 | 17.2 | 19.0 | 17.3 | 19.0 |
| PGM | Glycolysis/Gluconeogenesis | −44.3 | 18.8 | −42.3 | 18.4 | 16.5 | 18.4 | 16.6 | 18.8 |
| PPS | Glycolysis/Gluconeogenesis | 0.0 | 128.7 | 0.0 | 108.4 | 0.0 | 79.8 | 0.0 | 0.0 |
| PYK | Glycolysis/Gluconeogenesis | 0.0 | 144.2 | 0.0 | 112.3 | 3.5 | 92.7 | 4.6 | 16.6 |
| TPI | Glycolysis/Gluconeogenesis | −41.6 | 9.6 | −29.4 | 9.6 | 7.8 | 9.6 | 7.9 | 9.6 |
| ATPS4r | Oxidative phosphorylation | −24.2 | 108.4 | −24.2 | 106.6 | −24.2 | 60.3 | −24.2 | 63.5 |
| CYTBO3 | Oxidative phosphorylation | 27.9 | 77.2 | 31.1 | 77.2 | 31.1 | 43.7 | 31.1 | 44.2 |
| FDH2 | Oxidative phosphorylation | 0.0 | 27.1 | 0.0 | 27.1 | 0.0 | 15.3 | 0.0 | 15.3 |
| LDH_D2 | Oxidative phosphorylation | 0.0 | 68.7 | 0.0 | 54.5 | 0.0 | 0.0 | 0.0 | 0.0 |
| NADH10 | Oxidative phosphorylation | 0.0 | 68.7 | 0.0 | 58.8 | 0.0 | 0.0 | 0.0 | 0.0 |
| NADH12 | Oxidative phosphorylation | 0.0 | 68.7 | 0.0 | 54.8 | 0.0 | 35.0 | 0.0 | 42.1 |
| NADH6 | Oxidative phosphorylation | 0.0 | 68.7 | 0.0 | 53.7 | 0.0 | 35.0 | 0.0 | 42.1 |
| NADH7 | Oxidative phosphorylation | 0.0 | 68.7 | 0.0 | 58.8 | 0.0 | 0.0 | 0.0 | 0.0 |
| NADH8 | Oxidative phosphorylation | 0.0 | 68.7 | 0.0 | 58.8 | 3.0 | 41.5 | 0.0 | 0.0 |
| NADH9 | Oxidative phosphorylation | 0.0 | 68.7 | 0.0 | 58.3 | 0.0 | 0.0 | 0.0 | 0.0 |
| POX | Oxidative phosphorylation | 0.0 | 27.1 | 0.0 | 16.1 | 0.0 | 15.3 | 0.0 | 0.0 |
| SUCD4 | Oxidative phosphorylation | −0.1 | 77.2 | 2.3 | 60.4 | 5.0 | 43.7 | 0.3 | 44.2 |
| THD2 | Oxidative phosphorylation | 0.0 | 257.3 | 0.0 | 214.8 | 0.0 | 159.7 | 0.0 | 164.7 |
| THD5 | Oxidative phosphorylation | 0.0 | 272.6 | 0.0 | 229.6 | 0.0 | 163.9 | 0.0 | 169.9 |
| TRDR | Oxidative phosphorylation | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| EDA | Pentose Phosphate Cycle | 0.0 | 9.9 | 0.0 | 9.9 | 0.0 | 0.0 | 0.0 | 0.0 |
| G6PDHy | Pentose Phosphate Cycle | 0.0 | 9.9 | 0.0 | 9.9 | 0.0 | 5.5 | 0.0 | 5.2 |
| PGDH | Pentose Phosphate Cycle | 0.0 | 9.9 | 0.0 | 5.5 | 0.0 | 5.5 | 0.0 | 5.2 |
| PGDHy | Pentose Phosphate Cycle | 0.0 | 9.9 | 0.0 | 9.9 | 0.0 | 0.0 | 0.0 | 0.0 |
| PGL | Pentose Phosphate Cycle | 0.0 | 9.9 | 0.0 | 9.9 | 0.0 | 5.5 | 0.0 | 5.2 |
| RPE | Pentose Phosphate Cycle | −0.2 | 6.4 | −0.2 | 4.5 | −0.2 | 3.4 | −0.2 | 3.2 |
| RPI | Pentose Phosphate Cycle | −3.5 | −0.2 | −2.6 | −0.2 | −2.1 | −0.2 | −1.9 | −0.2 |
| TAL | Pentose Phosphate Cycle | −0.1 | 3.2 | −0.1 | 2.3 | −0.1 | 1.8 | −0.1 | 1.7 |
| TKT1 | Pentose Phosphate Cycle | −0.1 | 3.2 | −0.1 | 2.3 | −0.1 | 1.8 | −0.1 | 1.7 |
| TKT2 | Pentose Phosphate Cycle | −0.2 | 3.1 | −0.2 | 1.7 | −0.2 | 1.6 | −0.2 | 1.6 |
| ACt6 | Transport, Extracellular | −12.3 | 0.0 | −11.6 | 0.0 | −11.5 | −8.4 | −11.5 | 0.0 |
| GLCpts | Transport, Extracellular | 0.0 | 10.0 | 0.0 | 10.0 | 0.0 | 10.0 | 0.0 | 10.0 |
| GLCt2 | Transport, Extracellular | 0.0 | 10.0 | 0.0 | 10.0 | 0.0 | 10.0 | 0.0 | 10.0 |
| AMDNt | Amorphadiene | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| BIOMASS | Biomass | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
a
All flux values have units of mM/hr and are relative to a glucose uptake rate of 10 mM/hr.
b
The relaxed model only uses stoichiometric data and not the 13C labeling data and serves as the allowable bounds for the fluxes. Shown here is the relaxed model of the full model.
c
Flux abbreviations correspond to the names in (Reed et al. 2003) and are shown in Figure 6.