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Secondary structures of the fdhF mRNA hairpin (24) and selected aptamers according to chemical and enzymatic modification analyses. Protection of bases from chemical modification and S1 nuclease cleavage by SelB. ▾, Positions cleaved by S1 nuclease; •, indicates protection from S1 cleavage by SelB. Protection from chemical modification by SelB is shown by circled bases. Chemical and enzymatic probing of free RNA or RNA–SelB complexes was performed as described (24).
