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Samples of pacifastin (5 μg) were subjected to SDS/PAGE under nonreducing (lanes A, C, and E) or reducing (lanes B and D) conditions. The gels were Coomassie stained (lanes A and B) or counterstained for chymotrypsin inhibitory activity (lanes C, D, and E). The sample in lane E was preincubated with immobilized trypsin, and a 30-kDa fragment with strong chymotrypsin inhibitory activity is indicated by an arrow.
