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Specific inhibition of transcription elongation by psoralen adducts at the triplex site. Lane 1 (control), transcription without third strand. Lanes 2–4, increasing concentration of third-strand P15 without UV crosslinking. Lane 5, addition of 10 μM P15 and photocrosslinking reaction followed by transcription. Lane M is a 50-bp DNA marker, and nucleotide lengths (150 to 250) around the triplex site are shown.
