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Arrest and termination of RNA pol II elongation complexes by various oligonucleotide probes. Third-strand probes (10 μM) were added to the transcription reaction and UV irradiated as indicated (not isolated on magnetic beads). Lane M is a 50-bp DNA marker, and nucleotide lengths (150 to 250) around the triplex site are shown. Transcripts arising from transcription termination and arrest are indicated. Full-length transcripts produced from unmodified templates are also shown.
