CD of wtIL-5, DABC, and GM1. Thermal stability of IL-5 constructs was evaluated with a Jasco J-710 spectropolarimeter by monitoring the ellipticity at 222 nm in a 0.1 cm water-jacketed cuvette. Solid line, wtIL-5; dashed line, DABC; dotted line, GM1. (Inset) CD spectra at 25°C for the three forms of IL-5 as denoted in main figure. Conditions were the same as in the thermal stability measurements. Spectra shown are the average of three scans run at 50 nm per minute with a 2-sec response time.