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. 1997 Jun 24;94(13):6700–6705. doi: 10.1073/pnas.94.13.6700

Figure 4.

Figure 4

Effect of HIV NC on RNA hydrolysis and DNA strand transfer catalyzed by the p66/p51Δ13 RT. (A) Strand transfer reactions were completed as described with radiolabeled 40-nt RNA primary template (see Fig. 3A). RNase H hydrolysis products were resolved by electrophoresis on a denaturing 15% polyacrylamide gel. Reactions were completed in the presence (+NC) or absence (−NC) of the 71-amino acid form of HIV NC (10 μM). (B) Strand transfer reactions were performed as described with radiolabeled 20-nt DNA primer (see Fig. 3A). Quenched reactions were subjected to electrophoresis on a denaturing 15% polyacrylamide gel. Reactions were completed in the absence (−NC) or presence (+NC) of the 71-amino acid form of HIV NC (10 μM).