Figure 5.

Comparison of the effect of HIV NC and EIAV NC on RT-catalyzed DNA strand transfer. (A) Strand transfer reactions were performed as described using wild-type RT. The concentration of the 61-nt DNA strand transfer product is shown as a function of time for reactions completed in the absence of NC (▴), in the presence of 10 μM HIV NC (▪), and 10 μM EIAV NC (•). The plateau observed in the reaction with HIV NC is caused by activation of the synthesis-independent RNase H activity of HIV RT which depletes the system of 41-nt acceptor template (8). (B) Reactions were completed as described in A in the presence of 0.5 μM (•), 2 μM (▪), and 8 μM (▴) EIAV NC.