Table 1.
Comparison of polymerase and RNase H activities for wild-type (WT) and mutant RTs
Enzyme | Active enzyme, % | Polymerase activity, min−1 | RNase H activity, min−1 |
---|---|---|---|
WT | 56 ± 3 | 0.86 ± 0.05 | 1.04 ± 0.10 |
Δ5 | 52 ± 11 | 1.14 ± 0.07 | 2.70 ± 0.50 |
Δ9 | 28 ± 8 | 2.28 ± 0.09 | 3.10 ± 0.50 |
Δ13 | 42 ± 5 | 2.67 ± 0.13 | 2.50 ± 0.30 |