Figure 4.

Hypersensitivity to tetracycline and OmpF overproduction. (A) Five micrograms of total OMPs extracted from the strains C600 hupAB, C600, and C600 transformed by pompF grown in LB medium at 30°C, 37°C, and 42°C were analyzed by gel electrophoresis as described. (B) Five microliters of strains C600 (1), C600 hupAB (2), and C600 transformed by pompF (3) from an overnight culture in LB medium were spotted on LB plate with (+) or without (−) tetracycline (1 μg/ml) and incubated overnight at 30°C, 37°C, and 42°C.