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. 1997 Jun 24;94(13):6718–6723. doi: 10.1073/pnas.94.13.6718

Figure 4.

Figure 4

Activation of transcription by Drosophila RNA polymerase II. (A) Efficient activation by VP16–β in the presence of histone H1. Reaction mixtures containing 100 ng of nicked pLJ-17 DNA were assembled with 6.25 pmol of VP16–β and 3.1 pmol of γ complex (lanes 2, 5, 8, and 11), or with 200 ng of Gal4–VP16 (lanes 3, 6, 9, and 12). (B) Activation (in presence of 200 nM histone H1) requires the VP16 activation domain. Reaction mixtures contained nicked pLJ-17 DNA, 3.1 pmol of γ complex, and 6.25 pmol of either VP16–β (lane 2) or β (lane 3). (C) Activation requires nicked DNA. Components specified at the top of each lane were assembled as described in the text. Transcripts were detected by primer extension and quantified by phosphorimaging. Gal4–VP16 was generously provided by E. Blackwood (University of California at San Diego) and R. Hori (University of California at Los Angeles).