Figure 2.

pH_i measurement in isolated rabbit CEB. Dye-loaded CEB was initially perfused with Hepes buffer in the absence of CO₂. Perfusion buffer was changed from Hepes to bicarbonate containing CO₂, then to Cl⁻-free (gluconate) buffer. Preincubation of CEB with 1 mM amiloride or 2 μM bafilomycin A₁ was performed for 20 min at room temperature. A, B, and C are representative traces from control, amiloride-treated, and bafilomycin A₁-treated CEB, respectively.