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High-level expression of GFP-MNB/DYRK1A in multinucleate cells. HeLa cells were transfected with cDNA encoding GFP-MNB/DYRK1A. At 48 hours after transfection, the cells were fixed, stained with anti-MNB antibody and TOTO-3, and observed with a confocal laser scanning microscope as described in "Materials and Methods." Scale bar, 10 μm.
