Table 1.
Authenticity of the PCR products
| Clone | %AT | Southern blot | Genomic/cDNA |
|---|---|---|---|
| Hydra COUP | + | C | |
| Schistosoma TR4 | + | + | G |
| Schistosoma RXR | + | + | GC |
| Schistosoma FTZ-F1 | + | + | C |
| Schistosoma COUP | + | + | |
| Schistosoma COUPII | + | + | |
| Phallusia FTZ-F1 | G | ||
| Branchiostoma COUP | C | ||
| Branchiostoma TR2 | C | ||
| Danio REVERB | C | ||
| Danio COUP | C | ||
| Danio PPAR | C | ||
| Danio RAR | C | ||
| Danio NGFIB | C |
%A/T +: Sequence of the PCR fragment is rich in bases A and T, as is the genome of the corresponding organism. Southern blot +: Positive signal with the corresponding probe under high stringency conditions. G: Isolation of a genomic clone. C: Isolation of cDNA clones.