Table 1.
Gene conversion of leu2 alleles by a LEU2 fragment liberated in the nucleus or introduced by transformation
| Strain | Genotype difference | His+ total, % | His+ Leu+/ His+, % |
|---|---|---|---|
| A. HO-induced gene conversion of leu2-3,112 by LEU2 | |||
| G324 | No GAL::HO plasmid | 0.06 ± 0.04 | 0.06 ± 0.05 |
| G304 | 75.8 ± 11.6 | 0.012 ± 0.004 | |
| G366 | pms1Δ | 100 ± 11.6 | 0.07 ± 0.008 |
| B. HO-induced gene conversion of leu2-A by LEU2 | |||
| G487 | 100 ± 1 | 0.017 ± 0.002 | |
| G491 | pms1Δ | 87.5 ± 12 | 0.42 ± 0.042 |
| C. HO-induced gene conversion of leu2-3,112::ade1-E by LEU2::ADE1 | |||
| G523 | 89.6 ± 10.1 | 0.036 ± 0.003 | |
| G543 | pms1Δ | 97.7 ± 14.8 | 0.25 ± 0.047 |
| D. HO-induced gene conversion of leu2-K with homologous DNA ends | |||
| AM48 | 70.8 ± 16.5 | 0.030 ± 0.02 | |
| AM62 | Plus hcs::leu2-K::hcs* inserted at ADE1 | 88.4 ± 11.5 | 0.047 ± 0.02 |
| E. Transformation of linear LEU2 DNA and circular URA3-marked plasmid | |||
| Leu+/Ura+, % | |||
| G358 | 0.076 | ||
| G364 | pms1Δ | 0.26 | |
The relevant genotype of strains G304 and its derivatives is shown in Fig. 1A and are more fully described in Materials and Methods.
*
hcs = half of the HO endonuclease cleavage site (cs) homologous to the end of the HO-liberated LEU2 fragment.