Table 2.
LEU2 and ADE1 capture in pms1Δ strain G543
| Colony type | No. of His+ colonies |
|---|---|
| Leu+ Ade+/Leu+ Ade+ | 8 |
| Leu+ Ade+/Leu+ Ade− | 0 |
| Leu+ Ade+/Leu− Ade+ | 4 |
| Leu+ Ade+/Leu− Ade− | 7 |
| Leu+ Ade−/Leu− Ade+ | 0 |
| Leu+ Ade−/Leu+ Ade− | 6 |
| Leu+ Ade−/Leu− Ade− | 13 |
| Leu− Ade+/Leu− Ade+ | 11 |
| Leu− Ade+/Leu− Ade− | 16 |
An HO-liberated LEU2::ADE1 fragment could gene convert a leu2-3,112::ade1-E target locus to produce His+ colonies that were also Leu+ and/or Ade+ (Fig. 1B). In the experiment shown, 14,380 His+ colonies were examined. Each colony type is shown as two half-sectors. Those that are identical to the left and right of the slash were not sectored, while those that are nonidentical are colonies that were actually sectored.