Figure 1.

Extracts of total protein from lily primary microsporocytes at zygonema-pachynema (lanes 1 and 3) and tomato anthers containing primary microsporocytes at zygonema-pachynema (lanes 2 and 4) separated in one dimension and stained with Coomassie blue (lanes 1 and 2) or immunostained with affinity-purified polyclonal antibodies to full-length tomato Rad51 protein (lanes 3 and 4). In each immunoblot, the antibodies bind to a single protein band of approximately 40 kDa. From DNA sequence analysis, the predicted mass of tomato Rad51 protein is about 37 kDa (28). Molecular size standards are indicated on the left in kilodaltons.