Table 1.
Sarcoma cells expressing syngeneic MHC class II molecules are rejected, while sarcoma cells coexpressing class II plus accessory molecules are lethal in autologous and semi-syngeneic mice
| Tumor cells | Dose | Tumor incidence
|
|
|---|---|---|---|
| A/J | (C57BL/6 × A/J)F1 | ||
| SaI | 106 | 2/2 | 10/10 |
| SaI/Ak | 106 | 0/5 | 0/5 |
| SaI/Ak/Ii-6 | 106 | NT | 4/5 |
| SaI/Ak/Ii-7 | 106 | NT | 8/9 |
| SaI/Ak/Ii-9 | 106 | NT | 8/10 |
| SaI/LXSN | 106 | 5/5 | 5/5 |
| 5 × 105 | 3/5 | ||
| 105 | 1/5 | ||
| SaI/CIITA | 106 | 5/5 | 5/5 |
| 5 × 105 | 4/5 | ||
| 105 | 5/5 | ||
The indicated number of sarcoma cells were inoculated i.p. into autologous A/J or semisyngeneic (C57BL/6 × A/J)F1 mice, and the mice were followed for tumor incidence. NT, not tested. Institutional Animal Care and Use Committee guidelines mandate that moribund animals be sacrificed to minimize pain and discomfort. On the basis of previous studies, mean survival times of these mice would have been 14–19 days.