Table 2.
SaI/Ak, but not SaI/Ak/Ii or SaI/CIITA, tumor cells present peptides derived from ER-retained lysozyme
| APC (erHEL-transfected) | IL-2, pg/ml
|
|||||
|---|---|---|---|---|---|---|
| Exp. 1 | Exp. 2 | Exp. 3 | Exp. 4 | Exp. 5 | Exp. 6 | |
| SaI or SaI/LXSN | 0 | 0 | 0 | |||
| SaI/Ak | 79.2 | 47.5 | 85.8 | 594 | 198 | 99 |
| SaI/Ak/Ii-7 | 0 | 0 | 9.9 | |||
| SaI/Ak/Ii-9 | 0 | 0 | 0 | |||
| SaI/CIITA | 0 | 0 | 0 | |||
APC are transfectants expressing approximately equivalent levels of erHEL (see flow cytometry profiles in Fig. 1). APC were cocultured with 3A9 hybridoma cells, and IL-2 release was measured by ELISA. Six independent experiments are shown.