Table 3.
SaI/Ak and SaI/CIITA, but not SaI/Ak/Ii, cells effectively present membrane-associated HEL
| APC (mHEL-transfected) | IL-2, pg/ml
|
|||||
|---|---|---|---|---|---|---|
| Exp. 1 | Exp. 2 | Exp. 3 | Exp. 4 | Exp. 5 | Exp. 6 | |
| SaI or SaI/LXSN | 0 | 0 | 0 | |||
| SaI/Ak | 151.8 | 330 | 316.8 | 405 | 514.8 | 138.6 |
| SaI/Ak/Ii-6 | 6.6 | 0 | 0 | |||
| SaI/Ak/Ii-9 | 0 | 0 | 0 | |||
| SaI/CIITA 8.27 | 990 | >1,749 | ||||
| SaI/CIITA 15.11 | 1,056 | 798.6 | ||||
All APC are transfectants expressing approximately equal quantities of mHEL (see Fig. 1 for flow cytometry quantitation). APC were incubated with HEL-specific 3A9 T cell hybridoma cells, and IL-2 release was measured by ELISA. Six independent experiments are shown.