Figure 3.

Transport of class I heavy chains is inhibited in US6-transfected cells. US6 and vector control-transfected HeLaM cells were radiolabeled with [³⁵S]methionine and [³⁵S]cysteine for 15 min and chased for the indicated time periods. Triton X-100 lysates (1%) were immunoprecipitated with the mAb w6/32, digested or mock-digested with endo H, and subjected to 10% SDS/PAGE (A), and the ratio of endo H-resistant vs. endo H-sensitive class I heavy chains present at each time point quantitated by image analysis (B).