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. 1997 Jun 24;94(13):6904–6909. doi: 10.1073/pnas.94.13.6904

Figure 5.

Figure 5

US6 associates with the TAP/tapasin/class I complex. Digitonin lysates (1%) from US6 and control-transfected PaLa cells were immunoprecipitated with TAP1-specific (148.3) and control (κ immunoglobulin light chain-specific) mAb, coupled to A-15 m Sepharose beads. Precipitated proteins were eluted in 0.1% SDS/0.05% Triton X-100 buffer, separated by 12.5% SDS/PAGE under nonreducing conditions, transferred to Immobilon P membrane, and probed with TAP-specific, R.RING 4C (A), tapasin-specific, R.tapasinN (B), class I heavy chain-specific, 3B10.7 (C), and anti-US6 (D) antibodies.