Figure 1.

Vector construction. Schematic diagram of the plasmid DNA constructs used to make rAAV viruses mOp-lacZ (a) and mOp-gfp (b). TR, 145-bp AAV terminal repeat sequence; mOp, 472-bp murine rod opsin regulatory sequence from +86 to −385; SD/SA, 180-bp simian virus 40 late viral protein gene 16S/19S splice donor and acceptor signal; lacZ; coding sequence for the bacterial lacZ gene; gfp, coding sequence for the synthetic green fluorescence gene; pA, pA1, and pA2, polyadenylylation signals; Epo, a tandem repeat of the polyoma virus enhancer region (bases 5,210–5,274); Ptk, thymidine kinase promoter of herpesvirus (bases 92–218); neo^r, coding sequence of the neomycin resistance gene, Tn5 (bases 1,555–2,347) (22).