Table 1.
Titers of rAAV viruses and potential contaminants
Virus | rAAV titer, icu/ml | Adenovirus titer, pfu/ml | wtAAV titer, icu/ml |
---|---|---|---|
mOps-lacZ | 1 × 107 | Undetectable | <103 |
mOp-gfp | 4.6 × 109 | <103 | <103 |
rAAV and wtAAV were titered by infectious center assay. For wtAAV, 1 μl of the undiluted rAAV stock was titered (pfu, plaque-forming unit). Adenovirus was titered by plaque assay using 1 μl of the undiluted rAAV stock. No infectious adenovirus was detected by serial passage cytopathic effect.