Table 1.
Genetic background 129/SvJ × C57BL/6
| Day of gestation | Total | Genotype
|
||
|---|---|---|---|---|
| +/+ | +/− | −/− (%) | ||
| 9.5 | 63 | 11 | 37 | 15 (23.8) |
| 10.0 | 15 | 5 | 6 | 4 (26.7) |
| 10.5 | 54 | 11 | 33 | 10 (18.5) |
| 11.5 | 23 | 9 | 14 | 0 |
| 12.5 | 16 | 5 | 10 | 1 (6.2) |
| 13.5 | 69 | 23 | 45 | 1 (1.4) |
| 14.5 | 15 | 7 | 7 | 1 (6.7) |
| 15.5 | 21 | 9 | 11 | 1 (4.7) |
| 16.5 | 14 | 4 | 9 | 1 (7.1) |
| 17.5 | 37 | 8 | 27 | 2 (5.4) |
| 18.5 | 9 | 3 | 6 | 0 |
| P1-2 | 108 | 26 | 80 | 2 (1.9) |
| P21 | 303 | 109 | 194 | 0 |
| C-section | 42 | 18 | 23 | 1 (2.4) |
Genotype of offspring derived from timed matings of TF(+/−) hemizygotes. Genotypes were performed by Southern blot analysis of embryo, yolk sac, umbilical cord, or tail DNA using probe A. The morning on which a vaginal plug is identified is considered 0.5 days of gestation. P1-2 indicates 1-2 days postpartum. C-section, Cesarean section performed on E18.5.