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Genetic background 129/SvJ × 129/SvEv
| Day of gestation | Total | Genotype
|
||
|---|---|---|---|---|
| +/+ | +/− | −/− | ||
| E10.5 | 18 | 2 | 8 | 8 (44%) |
| E15.5 | 6 | 4 | 2 | 0 |
| E16.5 | 62 | 16 | 46 | 0 |
| E17.5 | 58 | 19 | 39 | 0 |
| E15.5–E17.5 | 126 | 39 | 87 | 0 |
| P21 | 82 | 25 | 57 | 0 |
Genotype of offspring derived from timed matings of TF(+/−) hemizygotes. Genotypes were performed by Southern blot analysis of embryo, yolk sac, or tail DNA using probe A.
