Figure 1.

RAD51-associated cDNA clones isolated by the yeast two-hybrid assay. (a) Predicted amino acid sequence of full-length RAB22, encoding 337 amino acids. No significant homology was observed to other sequences in GenBank. The GenBank accession number of the cDNA sequence is U93583. (b) Four representative cDNA fragments of RAB22 are shown as bold lines with the clone name listed on the right side. JG4-5–RAB22 was the original clone isolated by the yeast-two hybrid screening. RAB22-5 was cloned from a mouse thymus library using a cDNA probe prepared from JG4-5–RAB22, but contains a 56-nucleotide deletion (δ56) in the coding region. RAB22-5′-6 was fragment amplified from a mouse testis library by two primers (arrows), AP1 and RAB22-1R, and did not contain a deletion. RAB22 is the full-length cDNA clone constructed from RAB22-5 and RAB22-5′-6. The thin vertical lines are restriction enzyme sites as noted. (c) Relative position of the RAB163 peptide to the mouse BRCA2 (mBRCA2) peptide was shown by amino acid number and arrow. The full-length cDNA sequence of mBRCA2 has been reported (26).