Figure 1.

Analysis of genotypes by genomic PCR. Genomic DNA was prepared from the tails or livers of mice using the QIAamp tissue kit. PCRs were performed using a set of primers, W74, and W33 with genomic DNA as a template. The reaction products were electrophoresed on 5% or 8% polyacrylamide gels. (A) Schematic diagram of a part of the aggrecan gene containing exon 5. The expected sizes of the PCR products are indicated. (B) The gel electrophoresis patterns of the PCR products with DNA from a wild-type mouse (1), heterozygote (2), or homozygote (3). (C) The patterns of the gel electrophoresis of the PCR products after digestion by BpmI.