Fig. 3. Wild-type CFD1 restores glutamate-dependent growth to AR27/IRP1. Mutant strain AR27 or the cfd1-disrupted strain NS1 transformed with the indicated CFD1 allele carried on pRS313 was transformed with IRP1. Approximately 10⁴ transformed cells were spotted in 10 µl of water onto selective SD medium, with (+) or without (–) glutamate as indicated, and allowed to grow for 4 days at 30°C.