Fig. 7. Inhibition caused by the cfd1-1 mutation is specific to cytosolic Fe–S proteins. (A) Strains 0615d and AR27 were transformed with ACO1 and transformants were grown to mid-log phase in selective SD medium, harvested, and extracts were prepared for assay of aconitase (ACO1), succinate dehydrogenase (SDH), Leu1p, malate dehydrogenase (MDH) and NAD⁺-dependent isocitrate dehydrogenase (IDH) as described in Materials and methods. (B) Protein immunoblotting performed with 50 µg of total extract protein using antiserum specific for mitochondrial aconitase (Aco1p) or Leu1p.