Fig. 6. KLF11 downregulates oxidative stress scavengers. (A) KLF11 transgenic mice displayed a decrease in the expression of genes encoding key scavengers of oxidative stress such as SOD2 and Catalase1, as shown by array analysis and RT–PCR. (B) Western blot analysis shows a decrease in the protein levels of SOD2 and Catalase1 in KLF11 transgenic mice and PANC1 cells transfected with KLF11 (C) KLF11 overexpression in PANC1 results in the reduction of the promoter activity of both SOD2 and Catalase1. The mutant that lacks the Mad1-like SID motif loses the repressor activity on the promoters of oxidative stress scavengers. Control western blot shows expression of the KLF11 wild-type and mutant proteins. (D) Competition with Sp1 relieves the repression mediated by KLF11 compared with KLF11alone. Control western blot shows expression of the KLF11 wild-type protein. (E) Chromatin immunoprecipitation assay shows that KLF11 can bind the SOD2 and Catalase1 promoters in PANC1 cells. (F) TUNEL assay shows that the KLF11 animals were highly sensitive to the oxidative agent doxorubicin. (G) SOD2 and Catalase1 recombinant adenovirus are able to rescue the apoptotic phenotype induced by KLF11 and doxorubicin.