Fig. 6. Activated mN1IC accelerates expression of CD11b (Mac-1) in differentiating 32D cells. aIsotype controls of cells with or without OHT treatment were identical. Cells were induced to differentiate by removal of IL-3 and addition of G-CSF. On days 0, 4, 8 and 12, respectively, the cells were analysed by FACS analysis for expression of CD11b as a marker for granulocytic differentiation. On day 0, only cells without OHT treatment were analysed. By day 4 of differentiation in the presence of OHT, CD11b expression levels were equivalent to those seen after 12 days of differentiation in the absence of OHT. This experiment was repeated twice with the same results.