Table I. Activated mN1^IC enhances differentiation of 32D cells in the absence of G-CSF.

32D clone	Percentage of cell typesa			Total numberd of differentiated cells ×105	pe
	Blasts	EGb	LGc
rneo3
–OHT	99 ± 1	1 ± 1	0 ± 0	2 ± 2
+OHT	97 ± 1	3 ± 1	0 ± 0	5 ± 2
rNERTneo12
–OHT	100 ± 0	0 ± 0	0 ± 0	0 ± 0
+OHT	13 ± 1	56 ± 3	31 ± 4	48 ± 4	≤0.001
rNERTneo21
–OHT	95 ± 1	5 ± 1	0 ± 0	16 ± 3
+OHT	53 ± 2	44 ± 1	3 ± 1	42 ± 2	≤0.001

^aCells were cultured in low concentrations of IL-3 (1–10 U IL-3 per ml) for 7 days. Mean values ± SEM of two representative experiments using 2 U IL-3 per ml are shown. The experiment under low IL-3 conditions was repeated nine times yielding virtually identical results.

^bEarly granulocytes (promyelocytes and myelocytes).

^cLate granulocytes (metamyelocytes and granulocytes).

^dCalculated by multiplication of the absolute cell number with the percentage of differentiated cells (EG and LG).

^eThe increase in total number of differentiated cells after OHT treatment is statistically significant.