Fig. 8. In vitro interaction of wild-type and class II mutant proteins with Net1p. Binding of Myc epitope-tagged Sir2 proteins from whole-cell yeast extracts (wild type and class II mutants, as indicated) to GST–Net1(566–1189) protein bound to glutathione beads. Bound protein was detected by Western blotting using a monoclonal antibody against the Myc tag.