Table II. Yeast strains used in this study.
| Strain | Genotype | Reference |
|---|---|---|
| W303-1A | MATa ade2-1 trp1-1 can1-100 leu2-3.112 his3-11,15 ura3-1 | Thomas and Rothstein (1989) |
| GCY16 | MATa sir2::KanMX4 | this study |
| GCY23 | MATa RDN1::MET15 ΔAhmr::TRP1 adh4::URA3 Tel VIIL sir2Δ met15Δ ADE2 | this study |
| GCY40 | MATα RDN1::mURA3-HIS3 ΔAhmr::TRP1 adh4::ADE2 Tel VIIL sir2::KanMX4 | this study |
| GCY62 | MATα RDN1::4×UASg-mURA3-HIS3 sir2::KanMX4 | this study |
| GCY66 | MATα RDN1::MET15 ΔAhmr::TRP1 adh4::URA3 Tel VIIL met15Δ ADE2 | this study |
| GCY150 | MATa SIR3–GFP sir2::KanMX4 | this study |
| YG451 | MATa ΔAEhmr::3×UASg-TRP1 sir2::KanMX4 | Irina Serdobova |
| YG581 | MATa adh4::4×UASg-URA3 Tel VIIL sir2::KanMX4 | Irina Serdobova |
| CTY10-5D | MATa ade2-1 trp1-901 leu2-3 his3-200 gal4 gal80 URA3::lexA op-LacZ | Bartel and Fields (1995) |
All YG and GCY strains are isogenic derivatives of W303 (Thomas and Rothstein, 1989).