Table I. Spontaneous and UV-induced mitotic recombination frequencies.
Thiamine | UVa | Mean frequency of Ade+ recombinantsper 104 viable cells |
|||
---|---|---|---|---|---|
Deletiontype (His–) | Conversiontype (His+) | Total(His– + His+) | |||
Wild type | |||||
pREP1 | – | – | 1.97 | 0.72 | 2.69 |
+ | – | 1.73 | 0.75 | 2.48 | |
– | + | 1.12c | 1.09b | 2.21 | |
+ | + | 1.52 | 1.71b | 3.23 | |
pREP41-rus | – | – | 2.13 | 0.74 | 2.87 |
+ | – | 1.44 | 0.76 | 2.20 | |
– | + | 1.33c | 1.12b | 2.45 | |
+ | + | 0.87c | 1.53b | 2.40 | |
pREP1-rus | – | – | 4.63d | 1.40 | 6.03d |
+ | – | 2.43 | 0.76 | 3.19 | |
– | + | 5.60e | 1.57 | 7.17e | |
+ | + | 2.78 | 1.43b | 4.21 | |
pREP1-rus-D70N | – | – | 2.45 | 0.90 | 3.35 |
+ | – | 1.60 | 0.63 | 2.23 | |
– | + | 4.76f | 5.81g | 10.57e | |
+ | + | 1.26 | 3.52b | 4.78 | |
rqh1– | |||||
pREP1 | – | – | 5.24h | 0.91 | 6.15h |
+ | – | 4.25h | 1.66h | 5.91h | |
– | + | 23.24h | 9.75h | 32.99h | |
+ | + | 19.84h | 11.35h | 31.19h | |
pREP41-rus | – | – | 6.45 | 1.50 | 7.95 |
+ | – | 5.43 | 0.99 | 6.42 | |
– | + | 24.96 | 10.40 | 35.36 | |
+ | + | 26.29 | 11.77 | 38.06 | |
pREP1-rus | – | – | 12.50i | 1.48 | 13.98i |
+ | – | 7.36 | 2.01 | 9.37 | |
– | + | 44.50e | 8.85 | 53.35e | |
+ | + | 26.32 | 10.32 | 36.64 | |
pREP1-rus-D70N | – | – | 7.03 | 1.57 | 8.60 |
+ | – | 5.72 | 1.11 | 6.83 | |
– | + | 53.73e | 17.84j | 71.57e | |
+ | + | 20.15 | 8.27 | 28.42 |
a50 J/m2.
bIndicated value significantly (p <0.05) greater than –UV equivalent.
cIndicated value significantly (p <0.01) less than –UV equivalent.
dIndicated value significantly (p <0.0005) greater than pREP1, pREP1-resolvaseD70N and + thiamine equivalents.
eIndicated value significantly (p <0.0005) greater than pREP1 and + thiamine equivalents.
fIndicated value significantly (p <0.02) greater than pREP1 and + thiamine equivalents.
gIndicated value significantly (p <0.01) greater than all other wild-type conversion-type values.
hIndicated value significantly (p <0.0005) greater than wild-type equivalent.
iIndicated value significantly (p <0.03) greater than pREP1, pREP1-resolvaseD70N and + thiamine equivalents.
jIndicated value significantly (p <0.04) greater than all other rqh1– conversion-type values.