Fig. 3. Multilineage repopulation analysis of transplant recipients engrafted with cultured aorta- or UGR-derived HSCs. DNAs were isolated from hematopoietic tissue and sorted hematopoietic cells from representative transplant recipients receiving cells from (A) E11 cultured aorta and (B) E11 cultured UGRs. At >4 months post-transplantation, transplant recipients were killed and hematopoietic tissues isolated. Lymphoid, myeloid and erythroid lineage cells were sorted and DNAs examined for the presence of the donor cell genetic marker (human β-globin) as described in Figure 2.