Table III. HSC activity in co-cultured E11 aorta and UGR.
No. of mice repopulated/total transplanteda |
|||
---|---|---|---|
Marked tissue | Embryo equivalent | Cultured alone | Co-culturedb |
Aorta | 1 | 7/14 | 5/14 |
0.3 | 4/12 | 3/11 | |
UGR | 1 | 8/14 | 7/14 |
0.3 | 5/11 | 4/14 |
aOnly mice with ≥10% donor repopulation in peripheral blood at 4–6 months after transfer were included as positive. Data are from a total of five independent experiments.
bMarked aorta regions were cultured for 3 days with unmarked UGRs, and marked UGRs with unmarked aortas. Marked and unmarked co-cultured tissues were injected together.