Table IV. HSC activity in directly transplanted vitelline/umbilical arteries.
Embryo | No. of mice repopulated/total transplanteda |
|||
---|---|---|---|---|
Tissue | equivalent | E10.5 | E11.5 | E12.5 |
Vitelline/umbilical | 2.5–5 | 4/35b | ||
3 | 3/7 | 22/24 | ||
1 | 3/8 | 10/22 | ||
0.3 | 1/3 | 2/16 | ||
0.1 | 2/8 | |||
AGM | 2.5–3 | 1/12 | 16/16 | |
1 | 4/6 | 14/22 | ||
0.3 | 3/6 | 6/14 | ||
0.1 | 1/3 | 0/10 | ||
Liverc | 3 | 0/3 | 2/2 | |
1 | 2/6 | |||
0.3 | 1/2 |
aWith one exception (see below), only mice with ≥10% donor repopulation at 4–7 months after transfer were included as positive. Data are from a total of 12 independent experiments.
bOne mouse transplanted with 5 e.e. of umbilical arteries was engrafted at high levels (100%) at 3 months post-transplantation, but died before the >4 month bleed could be performed.
cLiver was used in these experiments as a positive control and thus is not mentioned in the text of the Results.