Fig. 1. Effects of gis1Δ, rim15Δ and gis1Δ rim15Δ on the expression of various genes following glucose starvation. Homozygous wild-type (YEF473), and gis1Δ/gis1Δ (CDV101), rim15Δ/rim15Δ (AR2) and gis1Δ/gis1Δ rim15Δ/rim15Δ (CDV104) mutant cells were harvested by centrifugation and resuspended in S medium containing 0.1% glucose. Total RNAs were extracted immediately after resuspension (1) and following further incubation for 90 (2), 180 (3) and 270 min (4). Equal amounts of RNAs (10 µg) were probed with SSA3, HSP12, HSP26, TPS2 and SSB1 fragments after electrophoresis and blotting. The application and transfer of equal amounts of RNA were verified by ethidium bromide staining.