Fig. 3. Immunoblot analysis of Gis1-myc13 in wild-type (NB14) and rim15Δ mutant (NB15) cells entering the diauxic transition phase. Samples were taken 2 h before glucose exhaustion (1), at the time of glucose exhaustion (2), and 2 (3), 4 (4) and 6 h (5) following glucose exhaustion. Twenty micrograms of extract protein were loaded in each lane.