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. 2000 Jun 1;19(11):2569–2579. doi: 10.1093/emboj/19.11.2569

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Fig. 3. Immunoblot analysis of Gis1-myc13 in wild-type (NB14) and rim15Δ mutant (NB15) cells entering the diauxic transition phase. Samples were taken 2 h before glucose exhaustion (1), at the time of glucose exhaustion (2), and 2 (3), 4 (4) and 6 h (5) following glucose exhaustion. Twenty micrograms of extract protein were loaded in each lane.