Fig. 5. Overexpression of GIS1, but not of RPH1, induces transcriptional activation of SSA3, HSP12 and HSP26. Wild-type (YEF473) cells transformed with either YCpADH1, YCpADH1-RIM15, YCpADH1-GIS1 or YCpADH1-RPH1 were grown to exponential phase on SD media containing 2% glucose. Total RNAs from exponentially growing transformants were extracted and equal amounts of RNAs (10 µg) were probed with SSA3, HSP12, HSP26, TPS2 and SSB1 fragments after electrophoresis and blotting. The application and transfer of equal amounts of RNA were verified by ethidium bromide staining.